Fluorescence enhancement of bis-acridine orange peptide, BAO, upon binding to double stranded DNA.
نویسندگان
چکیده
Bis-acridine orange peptides carrying two acridine oranges at the epsilon-amino moieties of both terminal lysines of a tetra(lysine) chain showed a ca. 200-fold fluorescence enhancement upon addition of double stranded DNA.
منابع مشابه
Human sperm bound to the zona pellucida have normal nuclear chromatin as assessed by acridine orange fluorescence.
BACKGROUND The aim of this study was to determine if only sperm with double stranded DNA could bind to the human zona pellucida (ZP). METHODS Sperm samples from 124 infertile men with a range of semen abnormalities were studied. Oocytes that had failed to fertilize in IVF or ICSI were used for the sperm-ZP binding test. A group of four oocytes were incubated for 2 h with 2 x 10(6)/ml motile s...
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• Acridine orange (A-1301, A-3568; Section 8.1) is one of the most popular and versatile fluorescent stains for histochemistry and cytochemistry and can provide a wide variety of information about the in situ content, molecular structure, conformation and environment of many nucleic acid–containing cell constituents.1 • Fluorescence photobleaching of DNA that has been photolytically labeled wit...
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ورودعنوان ژورنال:
- Organic & biomolecular chemistry
دوره 3 4 شماره
صفحات -
تاریخ انتشار 2005